: Know the Science behind detecting antibodies #IndiaNEWS #Education By Dr. Modala Mallesh Hyderabad: This article is in continuation of the previous articles that help you understand what constitutes

Know the Science behind detecting antibodies #IndiaNEWS #Education
By Dr. Modala Mallesh
Hyderabad: This article is in continuation of the previous articles that help you understand what constitutes the biological sciences and its varied branches/ concepts. Today, we would be discussing the fifth part of the Biomedical technology with a focus on Enzyme-linked immunosorbent assay (ELISA).
ELISA (Enzyme-linked immunosorbent assay)
? ELISA is the acronym (short form) of Enzyme-linked immunosorbent assay.
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Note: Monoclonal antibodies are used as
1. Primary antibodies (which react with the antigens of interest).
2. Secondary antibodies (which react with the primary antibodies).
ELISA in clinical immunology:
ELISA is a fundamental tool of clinical immunology, and the purpose of an ELISA is to determine if a particular protein is present in a sample, such as serum of a person affected by pathogen and if to how much.
Types of ELISA:
ELISA is of two types:
a. Direct ELISA- ELISA used to detect antigens
b. Indirect ELISA – ELISA done to detect antibodies.
Direct ELISA:
? Direct ELISA is performed by using primary antibodies (antibodies against the antigen of interest) attached/ adsorbed on the solid surface of the well on the titre plate.
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